Open AccessA ligase ribozyme obtained from a structured pool
Author(s) -
Wataru Yoshioka
Publication year - 2004
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/48.1.209
Subject(s) - ribozyme , rna , hairpin ribozyme , computational biology , ligase ribozyme , rna ligase , mammalian cpeb3 ribozyme , nucleic acid structure , vs ribozyme , sequence (biology) , folding (dsp implementation) , nucleotide , chemistry , biology , biochemistry , gene , engineering , electrical engineering
Here we report the in vitro selection experiment employed to construct a catalytic site on a self-folding RNA. To practice the selection, the sequence of 30 nucleotides was located in close proximity of the putative reaction site in a derivative of a naturally occurring RNA with a well known 3D structure. The acquired catalytic RNA retained the starting 3D structure and was successfully reconstructed according to its modular structure.
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