Mutational analyses of neighboring domains of active center of RNase P ribozyme | Zendy

Tatsuki Tanaka | Zendy

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Mutational analyses of neighboring domains of active center of RNase P ribozyme

Author(s) -

Tatsuki Tanaka

Publication year - 2004

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/48.1.201

Subject(s) - ribozyme , cleavage (geology) , rnase p , rnase h , vs ribozyme , ribonuclease , enzyme , chemistry , ribonuclease iii , mammalian cpeb3 ribozyme , escherichia coli , biology , genetics , microbiology and biotechnology , biochemistry , rna , gene , paleontology , fracture (geology) , rna interference

We prepared series of P2-P4 domain variants of Escherichia coli ribonuclease P (RNase P) ribozyme, and examined enzymatic activities of them. The results indicated that mutations in these domains did not affect the cleavage site selection of the enzyme, but affected cleavage efficiencies and dependence on magnesium ion concentrations.

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