Preparation and enzymatic recognition of guanine lesions induced by nitrogen oxides | Zendy

Akira Masaoka | Zendy; Hiroaki Terato | Zendy; Akiko Honsho | Zendy; Y. Ohyama | Zendy; Tetsuya Suzuki | Zendy; Masaki Yamada | Zendy; Keisuke Makino | Zendy; Hiroshi Ide | Zendy

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Preparation and enzymatic recognition of guanine lesions induced by nitrogen oxides

Author(s) -

Akira Masaoka,

Hiroaki Terato,

Akiko Honsho,

Y. Ohyama,

Tetsuya Suzuki,

Masaki Yamada,

Keisuke Makino,

Hiroshi Ide

Publication year - 2000

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/44.1.87

Subject(s) - guanine , deamination , xanthine , chemistry , oligonucleotide , dna glycosylase , dna , deoxyribonucleoside , enzyme , biochemistry , purine , nucleotide , hypoxanthine , polymerase , dna damage , gene

Xanthine (Xan) and oxanine (Oxa) are the major deamination products of guanine formed by the treatment with nitrogen oxides (e.g., NO and HNO2). In this study, 2'-deoxyribonucleoside 5'-triphosphates of Xan and Oxa were prepared by the NaNO2 treatment of dGTP. These modified nucleotides were incorporated into oligonucleotides by DNA polymerase reactions. The repair activities of various DNA N-glycosylases for Xan and Oxa were examined using these substrates.

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