Threonyl-tRNA synthetase of archaea: Importance of the discriminator base in the aminoacylation of threonine tRNA | Zendy

Hisayuki Ishikura | Zendy; Yoshiyuki Nagaoka | Zendy; Junji Yokozawa | Zendy; Tadashi Umehara | Zendy; Atsushi Kuno | Zendy; Taro Hasegawa | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Threonyl-tRNA synthetase of archaea: Importance of the discriminator base in the aminoacylation of threonine tRNA

Author(s) -

Hisayuki Ishikura,

Yoshiyuki Nagaoka,

Junji Yokozawa,

Tadashi Umehara,

Atsushi Kuno,

Taro Hasegawa

Publication year - 2000

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/44.1.83

Subject(s) - aminoacylation , haloferax volcanii , transfer rna , threonine , escherichia coli , biology , biochemistry , archaea , base pair , serine , enzyme , rna , dna , gene

To investigate the contribution of the discriminator base of archaeal tRNA(Thr) in aminoacylation by threonyl-tRNA synthetase (ThrRS), cross-species aminoacylation between Escherichia coli and Haloferax volcanii, halophilic archaea, was studied. It was found that E. coli ThrRS threonylated the H. volcanii tRNA(Thr) but that E. coli threonine tRNA was not aminoacylated by H. volcanii ThrRS. Results of a threonylation experiment using in vitro mutants of E. coli threonine tRNA showed that only the mutant tRNA(Thr) having U73 was threonylated by H. volcanii ThrRS. These findings indicate that the discriminator base U73 of H. volcanii tRNA(Thr) is a strong determinant for the recognition by ThrRS.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research