Open AccessMutagenicity of 5-formyluracil in mammalian cells
Author(s) -
Hiroyuki Kamiya,
N. Karino,
Yoshihito Ueno,
Akira Matsuda,
Hiroshi Kasai
Publication year - 2000
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/44.1.81
Subject(s) - thymine , simian , replication (statistics) , dna , dna replication , biology , base pair , mutation , vector (molecular biology) , origin of replication , genetics , stereochemistry , microbiology and biotechnology , chemistry , virology , gene , recombinant dna
5-Formyluracil, a major oxidized form of thymine, was incorporated into a predetermined site of one of the leading and lagging template strands of a double-stranded vector, and the DNA replication efficiency and the mutation frequency of 5-formyluracil in simian COS-7 cells were investigated. 5-Formyluracil did not block DNA replication and was weakly mutagenic in simian cells. 5-Formyluracil primarily elicited base substitutions at the modified positions.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom