Open AccessDevelopment of a novel functional biosensor with a short Ca2+-dependent deoxyribozyme
Author(s) -
Yasuhide Okumoto,
Naoki Sugimoto
Publication year - 2000
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/44.1.79
Subject(s) - deoxyribozyme , rna , biosensor , substrate (aquarium) , chemistry , cleavage (geology) , biochemistry , enzyme , biophysics , biology , dna , gene , ecology , paleontology , fracture (geology)
We develop a novel functional biosensor on a deoxyribozyme. A 5'-end-immobilized short Ca(2+)-dependent deoxyribozyme (dCGCTGGCAGGCTACAACGAGTCTTC) binds to a target RNA substrate (rGAAGACA decrease UGCCAGCG; decrease denotes an RNA cleavage site), and acts as an enzyme in the presence of Ca2+. It cleaves the target RNA substrate at one site of rAp decrease U in the asymmetric internal loop.
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