DNA lesion and mutagenesis induced in phageM13mp2 by UVA, UVB and UVC irradiation | Zendy

Yuji Izumizawa | Zendy; SongJu Yang | Zendy; Tomoe Negishi | Zendy; Kazuno Negishi | Zendy

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DNA lesion and mutagenesis induced in phageM13mp2 by UVA, UVB and UVC irradiation

Author(s) -

Yuji Izumizawa,

SongJu Yang,

Tomoe Negishi,

Kazuno Negishi

Publication year - 2000

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/44.1.73

Subject(s) - pyrimidine dimer , mutagenesis , dna , carcinogen , dna damage , mutation , microbiology and biotechnology , mutant , lesion , biology , genetics , chemistry , gene , medicine , pathology

Sunlight is carcinogenic and mutagenic and its genotoxic effects are believed to be the result of UV light-induced lesions in DNA. These lesions include pyrimidine dimers and (6-4) photoproducts, but it is uncertain whether the pyrimidine modifications are the sole pre-mutagenic lesions induced by UV light. Previous studies indicate that some sunlight-induced mutations in the single-stranded DNA phage M13mp2 may not be caused by these photoproducts. In this work, purified single-stranded phage DNA was exposed to UVA, UVB and UVC and the induced mutations were analyzed. All 3 types of UV light increase the mutation frequency. The mutants were sequenced and the results suggest that UVA exposure may induce formation of a non-dipyrimidine lesion in DNA.

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