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Covalent dimers of ImPyLDu86 with various linkers were synthesized and their DNA interstrand cross-linking abilities were examined. Denaturing polyacrylamide gel electrophoresis indicated that dimer molecule with tetramethylene linker efficiently produced interstrand cross-link only in the presence of appropriate partner PyIm triamides.

The synthesis of pyrrole(Py)/imidazole(lm) polyamide CPI conjugates which possess DNA interstrand cross-linking activity