Open AccessFluorescent probe and permeability to cells of isopoly (S-carboxymethyl-L-cysteine) derivative of nucleic acid bases
Author(s) -
Satoshi Kitaoka,
N. Tohnai,
Yoshiaki Inaki,
Toshihisa Hatae,
Takayuki Tanabe
Publication year - 1999
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/42.1.91
Subject(s) - nucleic acid , fluorescence , cysteine , chemistry , dna , transfection , oligomer , liposome , in vitro , biochemistry , biophysics , biology , organic chemistry , quantum mechanics , enzyme , gene , physics
Isopoly(S-carboxymethyl-L-cysteine) derivatives of nucleic acid bases were found to form stable complex with oligo-DNA in vitro. Fluorescent probed isopoly(S-carboxymethyl cysteine) derivatives of nucleic acid bases were prepared as antisense oligomers. The transfection of the oligomer into cells was carried out by HVJ-liposome method. Fluorescence was observed from the cells treated with HVJ-liposome including fluorescent probed oligomers.
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