Open AccessCleavage of parallel-stranded DNA duplex by peplomycin metal complexes
Author(s) -
Shuji Ikeda,
I. Saito,
H. Sugiyama
Publication year - 1999
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/42.1.41
Subject(s) - duplex (building) , cleavage (geology) , dna , metal , chemistry , combinatorial chemistry , materials science , biochemistry , organic chemistry , composite material , fracture (geology)
Peplomycin-mediated degradation of parallel-stranded (ps) duplex was investigated. It was found that Co- and Fe-peplomycins degraded ps DNA duplex by 4'-hydrogen abstraction at 5'-GPy (pyrimidine) site in a similar manner to that of antiparallel B-DNA. While the orientation of two strands of ps and B-form DNA duplexes are reversed, peplomycin metal complex can bind to ps DNA duplex to cause oxidative DNA damage. These results indicate that peplomycin metal complex mainly interacts with one strand which is damaged.
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