Open AccessComparative study of two ribozymes and DNA-enzyme against the same RNA target
Author(s) -
O. Inui,
Yosuke Kikuchi,
Natsuki Dohi,
Naoko Okada,
Hiroshi Okada,
Tatsuki Tanaka
Publication year - 1999
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/42.1.217
Subject(s) - ribozyme , ligase ribozyme , rna , enzyme , hammerhead ribozyme , enzyme kinetics , kinetics , dna , hairpin ribozyme , cleave , chemistry , microbiology and biotechnology , biochemistry , biology , gene , active site , physics , quantum mechanics
Two ribozymes, hammerhead ribozyme and hairpin ribozyme, and a DNA-enzyme were designed to cleave a same RNA target, the same site of the rat complement regulatory factor 512 antigen mRNA. The kinetic properties of these RNA-cleaving enzymes were measured and compared under the same conditions, using multiple turnover kinetics and competition kinetics. The catalytic efficiencies of these enzymes, and also the order of these enzymes will be discussed.
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