Highly enhanced duplex stability of dipyrido [3,2-a:2',3'-c] phenazine-modified oligonucleotide conjugate | Zendy

Yusuke Kitamura | Zendy; Toshihiro Ihara | Zendy; Yoshinori Shirasaka | Zendy; Tateishi Mitsuru | Zendy; Masato Tazaki | Zendy; Akinori Jyo | Zendy

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Highly enhanced duplex stability of dipyrido [3,2-a:2',3'-c] phenazine-modified oligonucleotide conjugate

Author(s) -

Yusuke Kitamura,

Toshihiro Ihara,

Yoshinori Shirasaka,

Tateishi Mitsuru,

Masato Tazaki,

Akinori Jyo

Publication year - 2003

Publication title -

nucleic acids symposium series

Language(s) - Uncategorized

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/3.1.95

Subject(s) - phenazine , conjugate , duplex (building) , oligonucleotide , chemistry , dna , stereochemistry , combinatorial chemistry , crystallography , biophysics , biology , biochemistry , mathematical analysis , mathematics

Dipyrido [3,2-a:2',3'-c] phenazine (DPPZ) or 1,10-phenanthroline (Phen) was tethered to the 5'-end of a short oligonucleotide (ODN) to generate two ODN conjugates. The conjugates formed stable duplexes with complementary 6 mer (d(TTAGGG)), which is one unit of telomeric repeats of human. The melting temperature of the duplex with DPPZ conjugate was higher than that of the corresponding duplex with unmodified 6 mer by 19.6 degrees C. This stabilization is enormous compared with those observed in other ODN conjugates reported previously. It would be attributed to the effective interaction of tethered heteroaromatic groups with DNA base stack of the duplex.

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