Simultaneous incorporation of three different modified nucleotides during PCR | Zendy

Masayasu Kuwahara | Zendy; Shin-ichi Hososhima | Zendy; Yoshinori Takahata | Zendy; Rina Kitagata | Zendy; Atsushi Shoji | Zendy; Kazuo Hanawa | Zendy; Akiko Ozaki | Zendy; Hiroaki Ozaki | Zendy; H. Sawai | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Simultaneous incorporation of three different modified nucleotides during PCR

Author(s) -

Masayasu Kuwahara,

Shin-ichi Hososhima,

Yoshinori Takahata,

Rina Kitagata,

Atsushi Shoji,

Kazuo Hanawa,

Akiko Ozaki,

Hiroaki Ozaki,

H. Sawai

Publication year - 2003

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/3.1.37

Subject(s) - nucleotide , nucleoside , chemistry , polymerase , dna , dna polymerase , linker , biochemistry , microbiology and biotechnology , stereochemistry , biology , gene , computer science , operating system

Modified analogs of 2'-deoxycytidine triphosphates bearing (6-aminohexyl)carbamoylmethyl or 7-amino-2,5-dioxaheptyl linker at a C5 position were designed and synthesized. Both analogs were found to be good substrates for Vent(exo-) DNA polymerase during PCR, resulting in the corresponding full-length modified DNAs, respectively. Moreover, we have demonstrated simultaneous incorporation of three different modified nucleotides into a DNA strand by PCR using triphosphates of 5-(3-aminopropynyl)dUTP, 5-[(6-aminohexyl)carbamoylmethyl]dCTP and 2-amino-dATP (dDTP) or N6-methyl-dATP in place of the natural nucleoside triphosphates TTP, dCTP and dATP.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research