Application of recombination transfer to the cognate Bacillus subtilis genome | Zendy

S. Tomita | Zendy; Kenji Tsuge | Zendy; Yo Kikuchi | Zendy; Mitsuhiro Itaya | Zendy

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Application of recombination transfer to the cognate Bacillus subtilis genome

Author(s) -

S. Tomita,

Kenji Tsuge,

Yo Kikuchi,

Mitsuhiro Itaya

Publication year - 2003

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/3.1.295

Subject(s) - bacillus subtilis , genome , plasmid , biology , genetics , dna , whole genome sequencing , computational biology , gene , bacteria

Isolation of the designated genome region of Bacillus subtilis was investigated using a B. subtilis recombinational transfer (BReT) system. Two DNA sequences flanking the precise genome region are cloned in the BReT vector. The BReT plasmid recovered the predicted genome sequence as large as 100 kb with high fidelity. The result indicates that the BReT system originally developed to recover the non-cognate segments cloned in the B. subtilis genome vector can be applied to the cognate sequence.

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