Effective photo-regulation of transcription reaction by SP6 RNA polymerase with modified DNA tethering multiple azobenzenes | Zendy

Man Liu | Zendy; Hiroyuki Asanuma | Zendy; Makoto Komiyama | Zendy

Open Access

Effective photo-regulation of transcription reaction by SP6 RNA polymerase with modified DNA tethering multiple azobenzenes

Author(s) -

Man Liu,

Hiroyuki Asanuma,

Makoto Komiyama

Publication year - 2003

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/3.1.265

Subject(s) - transcription (linguistics) , rna polymerase ii , rna polymerase , chemistry , polymerase , dna , azobenzene , microbiology and biotechnology , rna , biophysics , promoter , biology , biochemistry , gene , gene expression , molecule , philosophy , linguistics , organic chemistry

Effective photo-regulation of transcription reaction by SP6 RNA polymerase (RNAP) was achieved with photo-responsive SP6 promoter tethering two azobenzenes. With one azobenzene in either TATA or RNAP binding region of the SP6 promoter, photo-regulation activity was very small. But when two azobenzenes were introduced into both regions, efficient photo-regulation of transcription was attained: transcription proceeded 3.5 fold faster under UV irradiation than under dark.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research