Open AccessA Direct and efficient synthesis method for dumbell-shaped linear DNA using PCR in vitro
Author(s) -
Masumi Taki,
Yoshio Kato,
Makoto Miyagishi,
Yoko Takagi,
Masayuki Sano,
Kazunari Taira
Publication year - 2003
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/3.1.191
Subject(s) - dumbbell , dna , in vitro , recombinant dna , chemistry , covalent bond , computational biology , microbiology and biotechnology , combinatorial chemistry , gene , biology , biophysics , biochemistry , organic chemistry , physiology
A linear, covalently-closed, dumbbell-shaped DNA vector including a transcription unit is known to have both biological stability and safety and is expected to be useful for gene therapy. We established an easy, quick, and large preparative synthetic method of modified- and unmodified-dumbbell DNA using an intramolecular cyclization at the DNA termini.
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