Tandem site-selective RNA scission utilizing acridine-DNA conjugates | Zendy

Akinori Kuzuya | Zendy; Ryo Mizoguchi | Zendy; Takashi Sasayama | Zendy; Makoto Komiyama | Zendy

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Tandem site-selective RNA scission utilizing acridine-DNA conjugates

Author(s) -

Akinori Kuzuya,

Ryo Mizoguchi,

Takashi Sasayama,

Makoto Komiyama

Publication year - 2003

Publication title -

nucleic acids symposium series

Language(s) - Uncategorized

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/3.1.167

Subject(s) - phosphodiester bond , oligonucleotide , acridine , rna , chemistry , dna , bond cleavage , nucleotide , microbiology and biotechnology , stereochemistry , biochemistry , biology , gene , organic chemistry , catalysis

Useful technique to clip designated short RNA fragments from long substrates has been prepared by combining oligonucleotides bearing two acridine groups and lanthanide(III) ions. The substrate RNA is site-selectively activated at two designated phosphodiester linkages by complementary bis-acridine-modified DNA, and is promptly cleaved by lanthanide(III) ions to produce short RNA fragment between the two scission sites. By applying this technique, efficient genotyping method for single nucleotide polymorphism (SNP) have been developed.

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