Fluoreometric behavior of a novel bis-acridine orange bound to double stranded DNA | Zendy

Shigeori Takenaka | Zendy; Yoshimasa Sakakibara | Zendy; Hiroyuki Ueyama | Zendy; Takahiko Nojima | Zendy

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Fluoreometric behavior of a novel bis-acridine orange bound to double stranded DNA

Author(s) -

Shigeori Takenaka,

Yoshimasa Sakakibara,

Hiroyuki Ueyama,

Takahiko Nojima

Publication year - 2003

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/3.1.151

Subject(s) - acridine orange , circular dichroism , chemistry , acridine , moiety , dna , fluorescence , stacking , stereochemistry , intramolecular force , intercalation (chemistry) , crystallography , biochemistry , organic chemistry , apoptosis , physics , quantum mechanics

Novel bis-acridine orange (1) was synthesized from Fmoc-Lys(Boc)-OH and Fmoc-Lys(AO)-OH (AO: acridine orange), with the 9-position of acridine orange (AO) linked to the epsilon-amino moiety of lysine, on the peptide synthesizer. Bis-acridine orange (1) yielded a very weak fluorescence in an aqueous media due to the intramolecular stacking, but its fluorescence was enhanced over 200-times upon binding to double-stranded DNA, irrespective of the DNA sequences. Circular dichroism (CD) spectra showed that 1 binds to double stranded DNA in its stacked conformation, concomitant with fluorescence enhancement.

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