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Novel bi-functional anthraquinone phosphoramidite reagent was prepared from anthraquinolylethylenediamine and 2,2-bis(hydroxymethyl)propionic acid. The amidite reagent was incorporated into the middle position of oligoDNA consisting of pyrimidine residues, exclusively. The resulted oligoDNA was examined as the triple helix forming oligonucleotide (TFO) targeting a homopurine tract of a double helical DNA possessing a single pyrimidine-gap sequence.

Synthesis of novel TFOs bearing an abasic portion and their triplex forming ability with a DNA duplex containing a pyrimidine-gapped polypurine strand