The role of nucleosome positioning in repression by the yeast 2/Mcm1p repressor | Zendy

Satoshi Saitô | Zendy; Shigenori Miura | Zendy; Yoshio Yamamoto | Zendy; H. Shindo | Zendy; Miho Shimizu | Zendy

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The role of nucleosome positioning in repression by the yeast 2/Mcm1p repressor

Author(s) -

Satoshi Saitô,

Shigenori Miura,

Yoshio Yamamoto,

H. Shindo,

Miho Shimizu

Publication year - 2002

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/2.1.93

Subject(s) - psychological repression , nucleosome , repressor , saccharomyces cerevisiae , microbiology and biotechnology , biology , gene , regulation of gene expression , genetics , gene expression , histone

In Saccharomyces cerevisiae, alpha 2/Mcm1p represses a-cell specific genes. Previous studies indicated that repression by alpha 2/Mcm1p is associated with organization of positioned nucleosomes in promoters of a-cell specific genes such as STE6 and BAR1. Here, we examined the role of nucleosome positioning in repression of STE6-lacZ by alpha 2/Mcm1p. We showed that insertion of an A34 sequence, which is shown to disrupt an array of positioned nucleosomes, affects STE6-lacZ expression. Furthermore, artificial recruitment of TBP is facilitated by the insertion of A34 to derepress STE6-lacZ expression. These findings support the model that nucleosome positioning plays a regulatory role in repression by alpha 2/Mcm1p.

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