tRNA Recognition by CCA-adding enzyme | Zendy

Yukihide Tomari | Zendy; Tsutomu Suzuki | Zendy; Takuya Ueda | Zendy

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tRNA Recognition by CCA-adding enzyme

Author(s) -

Yukihide Tomari,

Tsutomu Suzuki,

Takuya Ueda

Publication year - 2002

Publication title -

nucleic acids symposium series

Language(s) - Uncategorized

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/2.1.77

Subject(s) - transfer rna , enzyme , biochemistry , biology , cloning (programming) , sequence (biology) , stereochemistry , chemistry , computational biology , rna , gene , computer science , programming language

The CCA-adding enzyme [ATP (CTP): tRNA nucleotidyltransferase (EC. 2.7.7.25)] catalyzes the addition of the conserved CCA sequence to the 3'-terminus of tRNAs. It is known that CCA-adding enzymes from all three kingdoms recognize the elbow region of L-shape tRNA formed by the tertiary interaction between T-loop and D-loop. We here demonstrate that C. elegans mitochondrial CCA-adding enzyme has broad substrate specificity toward nematode mitochondrial tRNAs which lack entire T- or D-arms, by cloning and characterizing the recombinant protein.

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