Open AccessInvestigation of DNA strand exchange mechanisms mediated by cationic polymers
Author(s) -
Yu Sato,
W. J. Kim,
Sung Won Choi,
Minako Saito,
Arihiro Kano,
T. Akaike,
Atsushi Maruyama
Publication year - 2002
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/2.1.59
Subject(s) - cationic polymerization , copolymer , branching (polymer chemistry) , oligonucleotide , histone octamer , polymer , dna , duplex (building) , dextran , chemistry , hydrogen bond , nucleotide , combinatorial chemistry , polymer chemistry , materials science , organic chemistry , biochemistry , molecule , gene , nucleosome , histone
It was observed that conjugation of dextran for PLL provided PLL-g-Dex with new properties of enhancing and accelerating inter-nucleotide recognition (1, 2, 3). In this paper, we report the effects of cationic PLL-g-Dex copolymers on base-pair formation of self-complementary octamer oligonucleotide by using NMR and CD analysis. NMR studies revealed that PLL-g-Dex copolymer did not affect hydrogen-bondings between bases in DNA duplex. CD studies also supported inert nature of the copolymer upon hybrid structure.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom