RNA LEGO: Magnesium-dependent assembly of RNA building blocks through loop-loop interactions | Zendy

Satoru Horiya | Zendy; Xiangdong Li | Zendy; Gota Kawai | Zendy; Rie Saito | Zendy; Akira Katoh | Zendy; K. Kobayashi | Zendy; Kazuo Harada | Zendy

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RNA LEGO: Magnesium-dependent assembly of RNA building blocks through loop-loop interactions

Author(s) -

Satoru Horiya,

Xiangdong Li,

Gota Kawai,

Rie Saito,

Akira Katoh,

K. Kobayashi,

Kazuo Harada

Publication year - 2002

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/2.1.41

Subject(s) - rna , loop (graph theory) , circular dichroism , nucleotide , nucleic acid secondary structure , linker , base pair , nucleic acid structure , chemistry , polyacrylamide gel electrophoresis , intron , biology , crystallography , dna , biophysics , genetics , computational biology , biochemistry , gene , computer science , mathematics , combinatorics , enzyme , operating system

We describe the construction of nano-molecular assemblies using RNA building blocks the human immunodeficiency virus type 1 (HIV-1) dimerization initiation site (DIS) RNA, that forms stable base pairing through a magnesium-dependent loop-loop interaction ("kissing"). RNA building blocks containing two DIS or DIS-like hairpins connected by a two nucleotide linker self-assembled to form specific structures as observed by non-denaturing polyacrylamide gel electrophoresis (PAGE). Furthermore, observation of "real time" formation of the molecular assemblies by circular dichroism (CD) spectroscopy was attempted.

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