An unnatural base pair between imidazolin-2-one and 2-amino-6-(2-thienyl)purine in replication and transcription | Zendy

Ichiro Hirao | Zendy; Michiko Kimoto | Zendy; Toshiaki Mitsui | Zendy; Yoko Harada | Zendy; Tsuyoshi Fujiwara | Zendy; Akira Sato | Zendy; Shigeyuki Yokoyama | Zendy

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An unnatural base pair between imidazolin-2-one and 2-amino-6-(2-thienyl)purine in replication and transcription

Author(s) -

Ichiro Hirao,

Michiko Kimoto,

Toshiaki Mitsui,

Yoko Harada,

Tsuyoshi Fujiwara,

Akira Sato,

Shigeyuki Yokoyama

Publication year - 2002

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/2.1.37

Subject(s) - purine , pairing , base pair , chemistry , stereochemistry , dna , selectivity , transcription (linguistics) , template , enzyme , biochemistry , physics , nanotechnology , catalysis , materials science , superconductivity , linguistics , philosophy , quantum mechanics

Nucleosides of imidazolin-2-one (designated by z) were designed and synthesized as pairing partners of 2-amino-6-(2-thienyl)purine (designated by s). Previously, we developed an unnatural base pair between s and pyridine-2-one (designated by y), and polymerases specifically incorporated the substrate of y into DNA and RNA opposite s in templates. Although s was efficiently incorporated opposite y, A was also incorporated opposite y with high efficiency. The replacement of y by z effectively improved the incorporation selectivity of s. The incorporation efficiency of A opposite z decreased, but the efficient incorporation of s opposite z was maintained as compared to that of the s-y pairing.

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