A new strategy for the synthesis of oligodeoxynucleotides in the phosphoramidite method without base protection via phosphite intermediates | Zendy

A. Ohkubo | Zendy; Yuya Ezawa | Zendy; Kohji Seio | Zendy; M. SEKINE | Zendy

Open Access

A new strategy for the synthesis of oligodeoxynucleotides in the phosphoramidite method without base protection via phosphite intermediates

Author(s) -

A. Ohkubo,

Yuya Ezawa,

Kohji Seio,

M. SEKINE

Publication year - 2002

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/2.1.29

Subject(s) - phosphoramidite , chemistry , deoxyadenosine , base (topology) , nucleophile , combinatorial chemistry , biochemistry , catalysis , dna , oligonucleotide , mathematics , mathematical analysis

Deoxyadenosine and deoxycytidine have highly nucleophilic amino groups. Therefore, without base protection, the undesired N-phosphitylations of these amino groups occur in the previous phosphoramidite methods. Now, we report that these N-phosphitylations were considerably suppressed in our new coupling strategy using HOBt as a promoter capable of formation of phosphite-type intermediates.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research