Selection of RNA-binding peptides containing Arg-rich motif | Zendy

Naohiko Shimada | Zendy; Kiyoko Oniwa | Zendy; Reiko Iwase | Zendy; Tetsuji Yamaoka | Zendy; Akira Murakami | Zendy

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Selection of RNA-binding peptides containing Arg-rich motif

Author(s) -

Naohiko Shimada,

Kiyoko Oniwa,

Reiko Iwase,

Tetsuji Yamaoka,

Akira Murakami

Publication year - 2002

Publication title -

nucleic acids symposium series

Language(s) - Uncategorized

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/2.1.281

Subject(s) - rna , 23s ribosomal rna , peptide , ribosomal rna , amino acid residue , chemistry , amino acid , residue (chemistry) , biochemistry , biology , microbiology and biotechnology , peptide sequence , gene , ribosome

In order to search the RNA-binding peptides, selection from randomized peptides containing Arg-rich motif was carried out using rRNA-immobilized column. As a result of the selection for 16S- and 23S-rRNA from E. coli, six peptides were identified to be the rRNA-binding peptide by MALDI-TOF MS. These peptides contained R, N, Q, or G at three randomized positions. This result indicates that amino acids with a positive residue or an amide residue might be essential for peptides to recognize the RNA.

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