In vitro selection of RNA aptamers that bind to domain II of HCV IRES | Zendy

Koji Kikuchi | Zendy; K. Fukuda | Zendy; Tadashi Umehara | Zendy; Jae Yeon Hwang | Zendy; Atsushi Kuno | Zendy; Takema Hasegawa | Zendy; Shin-Ichi Nishikawa | Zendy

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In vitro selection of RNA aptamers that bind to domain II of HCV IRES

Author(s) -

Koji Kikuchi,

K. Fukuda,

Tadashi Umehara,

Jae Yeon Hwang,

Atsushi Kuno,

Takema Hasegawa,

Shin-Ichi Nishikawa

Publication year - 2002

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/2.1.267

Subject(s) - internal ribosome entry site , aptamer , rna , biotinylation , translation (biology) , computational biology , untranslated region , ribosome , microbiology and biotechnology , biology , systematic evolution of ligands by exponential enrichment , riboswitch , chemistry , non coding rna , messenger rna , genetics , gene

The translation of HCV starts at the internal ribosomal entry site (IRES) within the 5' untranslated region and domain II of IRES is essential for translation activity. However, the information of function is limited. We attempted to obtain RNA that bind HCV IRES domain II. Selected aptamers will give us much information, such as RNA-RNA interaction between IRES and other cellular RNAs as well as target sites for anti-HCV drugs. We developed a novel selection method using biotinylated DNA probe. This method can be applied to many RNAs and would identify the most suitable target position experimentally and simply. The binding kinetics of aptamers were analyzed by using Biacore. Furthermore, inhibition of translation by aptamers was analyzed.

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