Thermodynamic analysis of hybridization between POPNA and DNA | Zendy

Mizuki Kitamatsu | Zendy; Masanori Shigeyasu | Zendy; T. Okada | Zendy; Takashi Nakai | Zendy; Mikio Saitou | Zendy; Masahiko Sisido | Zendy

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Thermodynamic analysis of hybridization between POPNA and DNA

Author(s) -

Mizuki Kitamatsu,

Masanori Shigeyasu,

T. Okada,

Takashi Nakai,

Mikio Saitou,

Masahiko Sisido

Publication year - 2002

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/2.1.261

Subject(s) - enthalpy , dna , rna , nucleic acid , chemistry , entropy (arrow of time) , nucleic acid thermodynamics , thermodynamics , stereochemistry , biochemistry , base sequence , physics , gene

We have developed a new series of oxy-peptide nucleic acids with pyrrolidine rings of four different types of structural isomers (cis L, cis D, trans L, trans D configurations; POPNAs). Melting curves of adenine nanomers of POPNAs in the presence of the complementary DNA showed the highest Tm value for cis L-POPNA, but in the case of RNA, the highest Tm value was observed for trans L-POPNA. Thermodynamic parameters (delta H and delta S) of POPNA/DNA and POPNA/RNA hybridizations were evaluated from concentration dependence of Tm values. The POPNA/DNA and POPNA/RNA hybridizations were governed by entropy and enthalpy terms, respectively.

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