Regulation of gene expresssion by sequence-specific alkylating polyamide | Zendy

Takanori Oyoshi | Zendy; Wakana Kawakami | Zendy; Tetsuya Bando | Zendy; Akihiko Narita | Zendy; Hitoshi Sugiyama | Zendy

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Regulation of gene expresssion by sequence-specific alkylating polyamide

Author(s) -

Takanori Oyoshi,

Wakana Kawakami,

Tetsuya Bando,

Akihiko Narita,

Hitoshi Sugiyama

Publication year - 2002

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/2.1.259

Subject(s) - dna , transcription (linguistics) , microbiology and biotechnology , gene , oligonucleotide , coding region , biology , gel electrophoresis , alkylation , chemistry , biochemistry , philosophy , linguistics , catalysis

In order to investigate the inhibition of gene expression by a new type of hairpin polyamide-CPI conjugate 1, its ability to inhibit transcription in cell free system was investigated. Sequence-selective alkylation of double-stranded DNA by 1 was investigated by denaturing gel electrophoresis using 1000 bp DNA fragment which codes for green fluorescence protein (GFP) under the control of T7 promoter. Analysis of DNA sequence indicated that 1 alkylated predominantly at the site of 5'-AGTCA-3' in coding region of GFP. The transcript by T7 RNA polymerase using the alkylated DNA as a template was analyzed by PAGE. The results clearly indicate that 1 inhibits transcription by alkylation of coding region at a nanomolar concentration.

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