Extensive methylation of CpG island of CYP24 gene in osteoblastic ROS17/2.8 cells | Zendy

Yoshihiko Ohyama | Zendy; T. Kusada | Zendy; Takuma Yamasaki | Zendy; H. Ide | Zendy

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Extensive methylation of CpG island of CYP24 gene in osteoblastic ROS17/2.8 cells

Author(s) -

Yoshihiko Ohyama,

T. Kusada,

Takuma Yamasaki,

H. Ide

Publication year - 2002

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/2.1.249

Subject(s) - cpg site , methylation , calcitriol receptor , dna methylation , promoter , gene silencing , gene , microbiology and biotechnology , epigenetics of physical exercise , biology , gene expression , chemistry , genetics

CYP24 is a target gene of 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) and is induced in the cells expressing vitamin D receptor (VDR) in response to 1,25-(OH)2D3. The osteoblastic ROS17/2.8 cells abundantly express VDR and have been used for the promoter analysis of many of vitamin D3 target genes. However, unlike other cells, ROS cells did not induce CYP24 expression upon 1,25-(OH)2D3 treatment. It has been reported that the methylation of CpG islands of a promoter region is involved in gene silencing. Methylation analysis of the CYP24 gene revealed that the CpG island in 5' part of the transcription unit is extensively methylated. This result suggests that unresponsiveness of the CYP24 gene to 1,25-(OH)2D3 would result from the methylation of the promoter region.

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