Enzymatic incorporation of an unnatural base pair between 4-propynyl-pyrrole-2-carbaldehyde and 9-methyl-imidazo[(4,5)-b]pyridine into nucleic acids | Zendy

Toshiaki Mitsui | Zendy; Masao Kimoto | Zendy; Yoko Harada | Zendy; Aya Sato | Zendy; A. Kitamura | Zendy; Teresa To | Zendy; Ichiro Hirao | Zendy; Shigeyuki Yokoyama | Zendy

Open Access

Enzymatic incorporation of an unnatural base pair between 4-propynyl-pyrrole-2-carbaldehyde and 9-methyl-imidazo[(4,5)-b]pyridine into nucleic acids

Author(s) -

Toshiaki Mitsui,

Masao Kimoto,

Yoko Harada,

Aya Sato,

A. Kitamura,

Teresa To,

Ichiro Hirao,

Shigeyuki Yokoyama

Publication year - 2002

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/2.1.219

Subject(s) - propynyl , klenow fragment , chemistry , stereochemistry , nucleoside , pyrrole , substrate (aquarium) , pyridine , nucleic acid , nucleotide , combinatorial chemistry , enzyme , medicinal chemistry , organic chemistry , biochemistry , polymerase , biology , ecology , gene , exonuclease

A hydrophobic unnatural nucleoside of 4-propynylpyrrole-2-carbaldehyde (designated as Pa') was synthesized to improve its affinity with a pairing partner, 9-methyl-imidazo[(4,5)-b]pyridine (Q), in enzymatic incorporation. In single-nucleotide insertion experiments using the Klenow fragment, the substrate of Pa' (dPa'TP) was efficiently incorporated opposite Q in the template strand, as compared to the incorporation of pyrrole-2-carbaldehyde (dPaTP), which was previously developed.

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