A novel fluorescent nonnatural amino acid that can be incorporated into a specific position of streptavidin | Zendy

Masumi Taki | Zendy; Takahiro Hohsaka | Zendy; Hitoshi Murakami | Zendy; Atsushi Kuno | Zendy; Tatsuya Hasegawa | Zendy; Kazunari Taira | Zendy; Masahiko Sisido | Zendy

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A novel fluorescent nonnatural amino acid that can be incorporated into a specific position of streptavidin

Author(s) -

Masumi Taki,

Takahiro Hohsaka,

Hitoshi Murakami,

Atsushi Kuno,

Tatsuya Hasegawa,

Kazunari Taira,

Masahiko Sisido

Publication year - 2002

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/2.1.203

Subject(s) - streptavidin , fluorescence , biotin , amino acid , beta (programming language) , biotinylation , chemistry , mutant , transfer rna , alpha (finance) , stereochemistry , biochemistry , rna , physics , computer science , mathematics , construct validity , statistics , quantum mechanics , gene , programming language , psychometrics

A novel highly fluorescent nonnatural amino acid (beta-(N-methylanthraniloyl)-L-alpha,beta-diaminopropionic acid; nmaDap) was incorporated at the 120th position of streptavidin. The position was directed by a CGGG/CCCG four-base codon/anticodon pair. The incorporation efficiency and the biotin-binding ability of the nmaDap mutant were compared to those of beta-anthraniloyl-L-alpha,beta-diaminopropionic acid (atnDap).

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