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For electrochemical DNA sequencing, several redox-responsive uridines modified at C5 were synthesized and site-specifically incorporated into DNA. Self-assembled DNA monolayers were prepared on a gold electrode using the dsDNA containing modified uridines. By measuring differential pulse voltammetry (DPV) using DNA-modified electrodes, the electrochemical response was strongly affected by the site of the redox-responsive uridine in dsDNA.

Development of electrochemically gene-analyzing method using DNA-modified electrodes