RNA cleavage efficiency and catalytic turnover of an oligonucleotide-two copper complexes conjugate | Zendy

Satoshi Sakamoto | Zendy; Takashi Tamura | Zendy; Toru Furukawa | Zendy; Yasuo Komatsu | Zendy; Eiko Ohtsuka | Zendy; Mitsuru Kitamura | Zendy; H. Inoue | Zendy

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RNA cleavage efficiency and catalytic turnover of an oligonucleotide-two copper complexes conjugate

Author(s) -

Satoshi Sakamoto,

Takashi Tamura,

Toru Furukawa,

Yasuo Komatsu,

Eiko Ohtsuka,

Mitsuru Kitamura,

H. Inoue

Publication year - 2002

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/2.1.157

Subject(s) - conjugate , oligonucleotide , rna , chemistry , catalysis , cleavage (geology) , copper , combinatorial chemistry , terpyridine , oligomer , reducing agent , stereochemistry , dna , polymer chemistry , biochemistry , organic chemistry , biology , metal , mathematical analysis , paleontology , mathematics , fracture (geology) , gene

We synthesized a 2'-O-methyloligonucleotide with two terpyridine-Cu(II) complexes in the central part, and found that this antisense agent cleaves an RNA oligomer more efficiently than an analogous agent with one Cu(II) complex. Determination of the pH dependence of the activity revealed the existence of an optimum pH for the two complexes-agent, which may be a result of the cooperative action of the complexes. In addition, we demonstrated that the novel agent catalytically cleaved an excess of the RNA substrate.

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