Novel approach for SNP genotyping based on site-selective RNA scission | Zendy

Akinori Kuzuya | Zendy; Ryo Mizoguchi | Zendy; F. Morisawa | Zendy; Makoto Komiyama | Zendy

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Novel approach for SNP genotyping based on site-selective RNA scission

Author(s) -

Akinori Kuzuya,

Ryo Mizoguchi,

F. Morisawa,

Makoto Komiyama

Publication year - 2002

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/2.1.129

Subject(s) - genotyping , rna , snp genotyping , acridine , single nucleotide polymorphism , snp , chemistry , dna , microbiology and biotechnology , bond cleavage , substrate (aquarium) , genotype , genetics , biology , catalysis , biochemistry , gene , ecology

Novel genotyping method for single nucleotide polymorphisms (SNPs), based on site-selective RNA scission, has been developed. A substrate RNA is activated at two sites by complementary acridine-modified DNA having two acridine residues, and is site-selectively cleaved by metal ion catalyst to produce short RNA fragment containing the SNP site. Genotype of the substrate is accurately and easily determined by mass analysis of the fragment.

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