Chromatin structure of yeast minichromosomes containing triplet repeat sequences associated with human hereditary neurological diseases | Zendy

Mitsuhiro Shimizu | Zendy; Risa Fujita | Zendy; Naruya Tomita | Zendy; Hitoshi Shindo | Zendy; Robert D. Wells | Zendy

Open Access

Chromatin structure of yeast minichromosomes containing triplet repeat sequences associated with human hereditary neurological diseases

Author(s) -

Mitsuhiro Shimizu,

Risa Fujita,

Naruya Tomita,

Hitoshi Shindo,

Robert D. Wells

Publication year - 2001

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/1.1.71

Subject(s) - chromatin , yeast , genetics , biology , gene

Expansion of triplet repeat sequences such as (CTG)n, (CGG)n, and (GAA)n causes human genetic diseases. Since DNA is packaged into arrays of nucleosomes in eukaryotic cells, chromatin may be involved in the mechanism of triplet repeat diseases. To elucidate this issue, we have examined effects of triplet repeat sequences on the chromatin organization in vivo using well defined yeast minichromosomes. We show here that (CGG)12 disrupts an array of positioned nucleosomes, whereas (CTG)12 promotes the nucleosome formation. Thus, triplet repeat sequences can affect the chromatin organization in vivo, which may contribute to the triplet repeat expansion or alterations in the expression of genes associated with triplet repeat diseases.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research