Recognition of small molecules by a ribonucleopeptide | Zendy

M. Hagihara | Zendy; Takashi Morii | Zendy; Keisuke Makino | Zendy

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Recognition of small molecules by a ribonucleopeptide

Author(s) -

M. Hagihara,

Takashi Morii,

Keisuke Makino

Publication year - 2001

Publication title -

nucleic acids symposium series

Language(s) - English

Resource type - Journals

eISSN - 1746-8272

pISSN - 0261-3166

DOI - 10.1093/nass/1.1.7

Subject(s) - rna , peptide , protein subunit , biochemistry , binding site , binding domain , biology , rna binding protein , microbiology and biotechnology , chemistry , computational biology , gene

In this research, we have design an ATP binding domain that consists of an RNA subunit and a peptide subunit by means of structure-based design approach and in vitro selection method. The RNA subunit is designed to consist of two functional domains; an ATP binding domain with 20 randomized nucleotides and an adjacent stem region that serves as a binding site for the RNA-binding peptide. RNA-peptide receptors to ATP were isolated from a pool of different RNAs by selection with affinity column and enzymatic amplification. Effects of the RNA-binding peptide for the specific ATP binding to the selected RNA-peptide receptors are discussed.

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