Open AccessCharacterization and formation mechanism of water-insoluble DNA-matrix induced by UV irradiation
Author(s) -
M. Yamada,
S. Satoh,
Motoyoshi Nomizu,
Kousaku Ohkawa,
Hiroyuki Yamamoto,
Nobuyuki Nishi
Publication year - 2001
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/1.1.205
Subject(s) - ethidium bromide , acridine orange , intercalation (chemistry) , agarose , irradiation , dna , chemistry , nuclease , aqueous solution , agarose gel electrophoresis , matrix (chemical analysis) , oligonucleotide , photochemistry , nuclear chemistry , chromatography , biochemistry , organic chemistry , apoptosis , physics , nuclear physics
We have prepared water-insoluble and nuclease resistant DNA-matrixes by UV irradiation. The UV-irradiated DNA-matrix could effectively accumulate and condense harmful DNA-intercalating compounds, such as acridine orange (AO) and ethidium bromide (EB), from diluted aqueous solutions. The binding constant of AO and EB for UV-irradiated DNA were determined to be 1.0 (+/- 0.2) x 10(5) M-1 and 6.8 (+/- 0.3) x 10(4) M-1, respectively; values consisted with reported results for non-irradiated DNA. In addition, the agarose gel electrophoresis and AFM measurements indicate that DNA matrix forms an intermolecular cross-linking structure with the radical reaction. The UV-irradiated DNA-matrixes have potential uses as a biomaterial filter for the removal of harmful DNA intercalating compounds.
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