Open AccessA novel detection method for 2'-deoxyoxanosine
Author(s) -
Masaki Yamada,
H. Honjo,
Takuya Hasegawa,
E Morimoto,
Toshinori Suzuki,
Takashi Morii,
Keisuke Makino
Publication year - 2001
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/1.1.159
Subject(s) - in vivo , chemistry , fluorescence , dna , reagent , deoxyguanosine , high performance liquid chromatography , biochemistry , chromatography , biology , optics , physics , microbiology and biotechnology
2'-Deoxyoxanosine (dOxo) is a novel DNA lesion produced from 2'-deoxyguanosine by the treatment with nitrous acid and nitric oxide. However, there has been no direct evidence on the formation of dOxo in vivo so far. In order to detect dOxo in vivo, we have explored a sensitive detection method specific for dOxo by using a fluorescent labeling reagent and HPLC analysis. An efficient method to detect the dOxo formation in DNA is described.
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