Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces | Zendy

Gholam Khodakaramian | Zendy; Sarah Lissenden | Zendy; Bertolt Gust | Zendy; Laura Moir | Zendy; Paul A. Hoskisson | Zendy; Keith Chater | Zendy; Margaret C. M. Smith | Zendy

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Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces

Author(s) -

Gholam Khodakaramian,

Sarah Lissenden,

Bertolt Gust,

Laura Moir,

Paul A. Hoskisson,

Keith Chater,

Margaret C. M. Smith

Publication year - 2006

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gnj019

Subject(s) - streptomyces coelicolor , biology , cre recombinase , temperateness , streptomyces , bacteriophage , recombinase , recombinant dna , phagemid , microbiology and biotechnology , gene , genetics , recombination , bacteria , escherichia coli , transgene , genetically modified mouse

We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage fC31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or Pgl in S.coelicolor. Cre-phage was also used for marker deletion in other strains of S.coelicolor

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