Site-specific biotinylation of RNA molecules by transcription using unnatural base pairs | Zendy

K. Moriyama | Zendy; Michiko Kimoto | Zendy; Takeki Mitsui | Zendy; Shigeyuki Yokoyama | Zendy; Ichiro Hirao | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Site-specific biotinylation of RNA molecules by transcription using unnatural base pairs

Author(s) -

K. Moriyama,

Michiko Kimoto,

Takeki Mitsui,

Shigeyuki Yokoyama,

Ichiro Hirao

Publication year - 2005

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gni128

Subject(s) - biotinylation , biology , rna , aptamer , transcription (linguistics) , base pair , dna , t7 rna polymerase , microbiology and biotechnology , biochemistry , computational biology , bacteriophage , gene , linguistics , philosophy , escherichia coli

Direct site-specific biotinylation of RNA molecules was achieved by specific transcription mediated by unnatural base pairs. Unnatural base pairs between 2-amino-6-(2-thienyl)purine (denoted by s) and 2-oxo(1H)pyridine (denoted by y), or 2-amino-6-(2-thiazolyl)purine (denoted as v) and y specifically function in T7 transcription. Using these unnatural base pairs, the substrate of biotinylated-y (Bio-yTP) was selectively incorporated into RNA, opposite s or v in the DNA templates, by T7 RNA polymerase. This method was applied to the immobilization of an RNA aptamer on sensor chips, and the aptamer accurately recognized its target protein. This direct site-specific biotinylation will provide a tool for RNA-based biotechnologies

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research