Four new type I restriction enzymes identified in Escherichia coli clinical isolates | Zendy

J. K. A. Kasarjian | Zendy

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Four new type I restriction enzymes identified in Escherichia coli clinical isolates

Author(s) -

J. K. A. Kasarjian

Publication year - 2005

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gni114

Subject(s) - isoschizomer , biology , escherichia coli , plasmid , restriction enzyme , genome , bacteria , enzyme , genetics , enterobacteriaceae , methylation , transformation (genetics) , dna , dna methylation , microbiology and biotechnology , gene , biochemistry , gene expression

Using a plasmid transformation method and the RM search computer program, four type I restriction enzymes with new recognition sites and two isoschizomers (EcoBI and Eco377I) were identified in a collection of clinical Escherichia coli isolates. These new enzymes were designated Eco394I, Eco826I, Eco851I and Eco912I. Their recognition sequences were determined to be GAC(5N)RTAAY, GCA(6N)CTGA, GTCA(6N)TGAY and CAC(5N)TGGC, respectively. A methylation sensitivity assay, using various synthetic oligonucleotides, was used to identify the adenines that prevent cleavage when methylated (underlined). These results suggest that type I enzymes are abundant in E.coli and many other bacteria, as has been inferred from bacterial genome sequencing projects

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