Open AccessUse of a restriction enzyme-digested PCR product as substrate for helicase assays
Author(s) -
Jeong Hoon Shin
Publication year - 2005
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/gni009
Subject(s) - helicase , biology , rna helicase a , enzyme , dna , computational biology , restriction enzyme , biochemistry , genetics , gene , rna
DNA helicases play essential roles in many cellular processes. The currently available techniques to generate substrates for helicase assays are fairly complicated and need some expertise not available in all laboratories. Here, a PCR-based method to generate a substrate for a helicase assay is described, and its application for several archaeal, bacterial and viral enzymes is demonstrated
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