High-throughput polyribosome fractionation | Zendy

Yipeng Wang | Zendy; Steven Ringquist | Zendy; Ann H. Cho | Zendy; Gaëlle Rondeau | Zendy; John Welsh | Zendy

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High-throughput polyribosome fractionation

Author(s) -

Yipeng Wang,

Steven Ringquist,

Ann H. Cho,

Gaëlle Rondeau,

John Welsh

Publication year - 2004

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gnh077

Subject(s) - polysome , sedimentation , centrifuge , centrifugation , biology , ultracentrifuge , fractionation , differential centrifugation , translation (biology) , sucrose , sucrose gradient , sedimentation coefficient , chromatography , messenger rna , microbiology and biotechnology , biochemistry , ribosome , rna , chemistry , sediment , gene , physics , paleontology , membrane , nuclear physics , enzyme

Polyribosome sedimentation velocity centrifugation can be used to identify differential regulation of the translation of mRNAs. However, ultracentrifugation presents practical limitations on the number of sedimentation velocity gradients that can be run simultaneously. A method for sedimentation velocity analysis of polyribosomes is presented that is based on low-speed centrifugation of sucrose gradients prepared in deep 96-well plates, the advantage of which is that hundreds of polyribosome fractionations can be performed simultaneously in a tabletop centrifuge

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