Surfection: a new platform for transfected cell arrays | Zendy

F H Chang | Zendy; Chien Hsin Lee | Zendy; Ming Ta Chen | Zendy; Chun Chen Kuo | Zendy; Yi Lin Chiang | Zendy; C Hang | Zendy; Steve R. Roffler | Zendy

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Surfection: a new platform for transfected cell arrays

Author(s) -

F H Chang,

Chien Hsin Lee,

Ming Ta Chen,

Chun Chen Kuo,

Yi Lin Chiang,

C Hang,

Steve R. Roffler

Publication year - 2004

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gnh029

Subject(s) - biology , transfection , computational biology , microbiology and biotechnology , genetics , cell culture

[[abstract]]Efficient high-throughput expression of genes in mammalian cells can facilitate large-scale functional genomic studies. Towards this aim, we developed a simple yet powerful method to deliver genes into cells by cationic polymers on the surface of substrates. Transfection can be achieved by directly contacting nucleic acid-cell mixtures with the cationic substrates, e.g. polyethylenimine/collagen-coated wells. This single-step matrix-surface-mediated transfection method, termed 'surfection', can efficiently deliver multiple plasmids into cells and can successfully assay siRNA-mediated gene silencing. This technology represents the easiest method to transfer combinations of genes in large-scale arrays, and is a versatile tool for live-cell imaging and cell-based drug screening

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