Semiconductor nanocrystal probes for human metaphase chromosomes | Zendy

Xiao Yan | Zendy

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Semiconductor nanocrystal probes for human metaphase chromosomes

Author(s) -

Xiao Yan

Publication year - 2004

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gnh024

Subject(s) - cadmium selenide , fluorophore , fluorescence , nanocrystal , biology , metaphase , in situ , fluorescence in situ hybridization , streptavidin , materials science , optoelectronics , quantum dot , nanotechnology , optics , gene , genetics , chemistry , biotin , physics , chromosome , organic chemistry

To improve signal stability and quantitation, an optically stable, novel class of fluorophore for hybridization analysis of human metaphase chromosomes is demonstrated. Detection of hybridization sites in situ was based on fluorescence from streptavidin-linked inorganic crystals of cadmium selenide [(CdSe)ZnS]. Fluorescence of nanocrystal fluorophores was significantly brighter and more photostable than organic fluorophores Texas Red and fluorescein. Thus, semiconductor nanocrystal fluorophores offer a more stable and quantitative mode of fluorescence in situ hybridization (FISH) for research and clinical applications

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