Oligonucleotide-directed site-specific integration of high complexity libraries into ssDNA templates | Zendy

Monica Hale | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Oligonucleotide-directed site-specific integration of high complexity libraries into ssDNA templates

Author(s) -

Monica Hale

Publication year - 2004

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gnh021

Subject(s) - biology , oligonucleotide , phagemid , template , computational biology , restriction site , aptamer , restriction enzyme , genomic library , shuttle vector , peptide library , vector (molecular biology) , phage display , dna , microbiology and biotechnology , combinatorial chemistry , base sequence , genetics , peptide , biochemistry , peptide sequence , bacteriophage , computer science , gene , recombinant dna , programming language , escherichia coli , chemistry

We present an approach that generates an oligomer-based library with minimal need for restriction site modification of sequences in the target vector. The technique has the advantage that it can be applied for generating peptide aptamer libraries at sites within proteins without the need for introducing flanking enzyme sites. As an example we present a phagemid retroviral shuttle vector that can be used to achieve stable expression of the library in mammalian cells for the purpose of screening for peptides with desired biological activity

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research