Projector: automatic contig mapping for gap closure purposes | Zendy

Sacha A. F. T. van Hijum | Zendy

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Projector: automatic contig mapping for gap closure purposes

Author(s) -

Sacha A. F. T. van Hijum

Publication year - 2003

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gng144

Subject(s) - contig , biology , genome , lactococcus lactis , projector , computational biology , genetics , bacterial genome size , sequence assembly , computer science , gene , computer vision , bacteria , lactic acid , gene expression , transcriptome

Projector was designed for automatic positioning of contigs from an unfinished prokaryotic genome onto a template genome of a closely related strain or species. Projector mapped 84 contigs of Lactococcus lactis MG1363 (corresponding to 81% of the assembly nucleotides) against the genome of L.lactis IL1403. Ninety three percent of subsequent gap closure PCRs were successful. Moreover, a significant improvement in the N50 and N80 values (describing the assembly quality) was observed after the use of Projector. Because increasing numbers of bacterial genomes are being sequenced, Projector provides an efficient method to close a significant number of remaining gaps in the late stages of a genome sequencing project

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