Improved generation of recombinant baculovirus genomes in Escherichia coli | Zendy

Kari J. Airenne | Zendy

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Improved generation of recombinant baculovirus genomes in Escherichia coli

Author(s) -

Kari J. Airenne

Publication year - 2003

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gng102

Subject(s) - recombinant dna , biology , escherichia coli , transposition (logic) , vector (molecular biology) , baculoviridae , plasmid , genome , genetics , selection (genetic algorithm) , virology , recombinant virus , computational biology , gene , spodoptera , computer science , artificial intelligence

An improved method for the generation of recombinant baculoviruses by Tn7-mediated transposition is described. The method is based on the modified donor vector (pBVboost) and an improved selection scheme of the baculovirus bacmids in Escherichia coli with a mutated SacB gene. Recombinant bacmids can be generated at a frequency of ∼107/µg of donor vector with a negligible background. This easy-to-use and efficient pBVboost system provides the basis for a high-throughput generation of recombinant baculoviruses as well as a more convenient way to produce single viruses. The introduced selection scheme is also useful for the construction of other vectors by transposition in E.coli

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