Open AccessA simple two-step, 'hit and fix' method to generate subtle mutations in BACs using short denatured PCR fragments
Author(s) -
Ye Yang
Publication year - 2003
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/gng080
Subject(s) - biology , oligonucleotide , bacteriophage , nucleotide , recombinant dna , mutation , computational biology , genetics , dna , microbiology and biotechnology , gene , two step , escherichia coli , combinatorial chemistry , chemistry
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